Course Live Advanced Dr. Omics Edu

Gut Microbiome Bioinformatics: From Raw 16S Reads to Biomarker Discovery

A hands-on microbiome bioinformatics course covering the complete 16S rRNA sequencing workflow from raw reads and quality control to microbial profiling, diversity analysis, and biomarker discovery.

  • 5.0/5
  • English
  • Updated Oct 2026
INR

₹45000

₹60000 25% off
USD

$600

$700 14% off

Indian learners pay in INR; international learners are billed in USD.

Enroll for International Students

Paying from outside India? Use this link to complete your payment.

Make an Enquiry
Gut Microbiome Bioinformatics: From Raw 16S Reads to Biomarker Discovery

About this course

The Gut Microbiome Bioinformatics: From Raw 16S Reads to Biomarker Discovery at Dr. Omics Edu is a live, mentor-led online course in microbiome, metagenomics and transcriptomics. It follows a gut microbiome dataset from raw 16S reads through to candidate biomarkers. You learn on real biological data and finish with an omics dataset analysed from raw data to biological interpretation that you can show to an employer, a guide or an admissions panel.
In this course you move through microbiome study design and 16s sequencing basics, read quality control and denoising and taxonomic classification, then apply them in a hands-on project. The course runs live online across 1 module, so you can ask your mentor questions as you work. It is an advanced-level course, best suited to learners who already know the fundamentals.

What you will learn

• Microbiome study design and 16S sequencing basics
• Read quality control and denoising
• Taxonomic classification
• Alpha and beta diversity analysis
• Differential abundance and biomarker discovery
• Visualization and interpretation of results
Microbiome data analyst, metagenomics analyst, transcriptomics analyst, research associate.

Skills you will gain

Linux QIIME 2 DADA2 R LEfSe
Certification

Available

Issued by Dr. Omics Edu

Course curriculum

1 module

  • Day 1 – Introduction to microbiome studies and amplicon sequencing principles (16S/18S/ITS overview).
  • Day 2 – Understanding marker genes, primer design, and choosing the right amplicon target.
  • Day 3 – Introduction to QIIME 2: architecture, artifacts (.qza/.qzv), and data import.
  • Day 4 – Demultiplexing raw reads and initial quality assessment in QIIME 2.
  • Day 5 – Quality filtering and trimming strategies for amplicon data.
  • Day 6 – ASV generation with DADA2: denoising, error learning, and merging paired reads.
  • Day 7 – Alternative ASV/OTU approaches with Deblur and comparing DADA2 vs Deblur outputs.
  • Day 8 – Taxonomic classification using SILVA and Greengenes2 reference databases.
  • Day 9 – Building and interpreting taxonomy bar plots and feature tables.
  • Day 10 – Alpha diversity metrics: Shannon, Chao1, and observed features.
  • Day 11 – Beta diversity metrics: Bray-Curtis, UniFrac, and ordination (PCoA).
  • Day 12 – Statistical hypothesis testing: PERMANOVA and ANOSIM for group comparisons.
  • Day 13 – Differential abundance/biomarker discovery using ANCOM-BC.
  • Day 14 – Differential abundance with ALDEx2 and comparing compositional data approaches.
  • Day 15 – Microbiome data visualization in R using phyloseq (ordination plots, heatmaps, trees).

What you need to start

  • Basic understanding of microbiology, molecular biology, or genetics.
  • Basic knowledge of NGS concepts is recommended but not mandatory.
  • Basic computer skills.
  • Familiarity with Linux or command-line tools is helpful but not required.
  • No prior microbiome bioinformatics experience is required.

Who this course is for

  • B.Sc, M.Sc, B.Tech, M.Tech and PhD students in microbiology, biotechnology, genetics or life sciences.
INR

₹45000

₹60000 25% off
USD

$600

$700 14% off

Indian learners pay in INR; international learners are billed in USD.

Enroll for International Students

Paying from outside India? Use this link to complete your payment.

Make an Enquiry
Active batch
Open for enrolment
Targeted Metagenomics for Gut Microbiome
  • Starts 19 Oct 2026
  • Ends 06 Nov 2026
  • Timing 7:00 PM – 8:00 PM
  • Days Mon, Tue, Wed, Thu, Fri
  • Platform MS Teams
This course includes
  • Format Live
  • Level Advanced
  • Language English
  • Modules 1
  • Certificate Yes
  • Provider Dr. Omics Edu
  • Live
  • instructor-led interactive sessions.
  • Hands-on analysis of representative 16S rRNA sequencing datasets.
  • Step-by-step guidance through the microbiome bioinformatics workflow.
  • Practical exercises covering microbial profiling and diversity analysis.
  • Exposure to commonly used microbiome analysis tools and workflows.
  • Dataset and supporting learning resources.
  • Course materials for reference and practice.
  • Certificate of participation/completion as applicable.
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