Go from raw RNA-seq reads to a publication-ready volcano plot in one live, hands-on session — using Galaxy's point-and-click tools. No coding required.
No catch and no upsell to sit through: the 4-hour live session costs nothing. Add-ons below are for people who want a certificate, the recording, or a review of their own analysis.
No charges to attend the masterclass.
Something to show for it on your CV.
Everything you need to redo it on your own data.
For anyone taking this into a thesis or paper.
Each tier includes everything in the one before it. Add-ons are entirely optional — you can attend the full class without paying anything. See what we cover ↓
Not slides about theory — you'll run the real RNA-seq to DGE pipeline end to end, on a real dataset, inside Galaxy.
Inspect read quality, spot adapter contamination, and clean your reads before anything downstream.
Map cleaned reads to the genome and understand what a good alignment rate actually looks like.
Turn alignments into a gene-level count matrix — the input every DGE tool expects.
Compare conditions with DESeq2, and learn to read normalized counts, log₂ fold change, and adjusted p-values.
Build the volcano plot, MA plot, heatmap, and PCA — then make sense of which genes matter and why.
If you have RNA-seq data (or will soon) and don't want to learn R or Python just to analyze it, this is for you.
You generated the sequencing data. Now you want to analyze it yourself instead of waiting in a queue.
You need clean, defensible DGE results — and a workflow you can describe in your methods section.
You want a reproducible, shareable pipeline your whole team can run without touching a command line.
You've heard "differential expression" a hundred times and want to finally do it — start to finish.
Optional add-on from DrOmics Research & Education Lab — add it to your CV, LinkedIn, or lab profile.
DrOmics is a molecular diagnostics and bioinformatics lab first. The training exists because we kept meeting researchers stuck on data they couldn't analyze.
DrOmics was founded by a translational bioinformatician who earned her PhD at the International Centre for Genetic Engineering and Biotechnology (ICGEB) and worked as a senior bioinformatics scientist before starting the lab. She built DrOmics around a gap she saw repeatedly: capable life-science researchers sitting on sequencing data with no practical way to analyze it.
That's the gap this masterclass is designed to close. The session itself is run by the DrOmics bioinformatics training team.
"I'd been sitting on RNA-seq data for months, intimidated by the command line. In one session I had my own volcano plot. The Galaxy approach just clicked."
"The best part was understanding why each step exists, not just clicking buttons. I finally get log₂ fold change and adjusted p-values."
"I reran the whole workflow on my own thesis data the next week using the project template. Saved me from begging a bioinformatician for help."
No. Galaxy is entirely point-and-click through a web browser — that's the whole reason we teach differential expression this way. If you can use a spreadsheet, you can follow along.
Yes — attending the live masterclass costs nothing, and seats are capped at 100 so the session stays interactive. The add-ons build on each other: ₹199 adds a certificate, ₹599 adds the recording, study material and practice dataset on top of that, and ₹1,499 adds a project template and a 1:1 review on top of everything else. None are required to attend.
Nothing. Galaxy runs in your browser and is free to use. We'll walk you through creating an account before the session so you're ready to go.
No — we provide a sample RNA-seq dataset so everyone can practice on the same thing. If you already have your own data, you're welcome to bring it and apply the workflow afterward.
The live session is where the hands-on work happens, so come if you can. If you can't, the ₹599 add-on includes the recording, study material, and practice dataset so you can work through the pipeline at your own pace.
With the ₹1,499 add-on you get everything in the ₹599 bundle, plus a project template and one one-to-one session where a DrOmics bioinformatician reviews your own analysis — your thresholds, your plots, your interpretation.
Join the free live masterclass and leave with a volcano plot, a working Galaxy pipeline, and the confidence to run differential expression on your own data.
Master bioinformatics skills and become job ready with Dr Omics Edu.
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Dr. Omics Edu — Transforming Life Sciences
