Decode the Transcriptome: A Hands-On Galaxy RNA-Seq Workshop

Unlock the full potential of Next-Generation Sequencing (NGS) data with this comprehensive two-day bioinformatics workshop designed for life sciences professionals and researchers. Transcriptomics forms the backbone of modern molecular biology, enabling precise measurement of gene expression across diverse biological conditions. This hands-on course eliminates complex command-line programming barriers by utilizing the accessible, cloud-enabled Galaxy platform for end-to-end RNA-Seq data processing. Participants will gain practical expertise in evaluating raw FASTQ read quality, trimming low-quality sequences, and performing precise genomic alignment with industry-standard tools like HISAT2 and STAR. You will master quantification techniques to extract gene-level counts using FeatureCounts, followed by rigorous differential expression analysis via DESeq2 and EdgeR. Finally, learn how AI-driven bioinformatics tools enhance data interpretation by transforming statistical outputs into intuitive volcano plots and publication-grade heatmaps. Designed for immediate practical application, this course bridges the gap between raw sequencing outputs and actionable biological discovery.