Targeted Metagenomics for Gut Microbiome
Unlock the secrets of the human gut! This crash course is designed to take you from raw sequencing data to meaningful biological insights using Targeted Metagenomics. Whether you are a student or a professional, this course will equip you with the high-demand bioinformatics skills needed to navigate the "second genome" of the human body.
Course Description
The human gut microbiome is a powerhouse of health, influencing everything from immunity to mental health. This course provides a comprehensive deep dive into Targeted Metagenomics, specifically focusing on 16S rRNA amplicon sequencing.
Unlike broad shotgun approaches, targeted metagenomics is a cost-effective, high-resolution method for identifying microbial diversity. Participants will journey through the entire bioinformatics pipeline: from raw sequence quality control and chimera removal to taxonomic classification and advanced statistical visualization. You will work with real-world gut microbiome datasets to identify biomarkers for health and disease.
🌟 Why This Course?
Clinical Relevance: Learn how microbial dysbiosis links to IBD, obesity, and diabetes.
Industry Demand: Bioinformaticians with microbiome expertise are highly sought after in pharma, diagnostics, and AgTech.
Hands-on Learning: Move beyond theory with practical labs using industry-standard tools like QIIME2 and R.
What You'll Learn
By the end of this course, you will be able to:
Execute a complete 16S rRNA bioinformatics workflow from scratch.
Critically Evaluate the quality and biases of metagenomic sequencing data.
Generate publication-quality visualizations of microbial communities.
Interpret the functional implications of gut microbial shifts in clinical contexts.
Curriculum
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"1= Introduction to metagenomics
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2= Tools installation for metagenomics
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3= Data Downloading
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4= Quality control & Trimming
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5= Data importing in Qimme2
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6= Data quality check using DADA2
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7= Phylogentic Analysis
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8= Taxonomy Analysis
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9= Krona Plot
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0= Phylogenetic tree construction"
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