Bootcamp Recording Available All Levels Dr. Omics

Galaxy RNA-Seq Bootcamp: Turn Sequencing Data into Discoveries

Master real-time gene quantification and molecular data analysis in just three days. Elevate your life science research career with the ultimate hands-on qPCR toolkit.

  • 5.0/5
  • English
  • Updated Jun 2026
Galaxy RNA-Seq Bootcamp: Turn Sequencing Data into Discoveries

About this course

Unlock the power of molecular diagnostics with The Gene Expression Toolkit, an intensive 3-day crash course engineered for modern life scientists. Spearheaded by Dr. Shweta Goyal, PhD (Postdoctoral Researcher at the National Brain Research Centre), this program bridges the gap between raw biological data and actionable genomic insights. Participants will master qPCR (quantitative PCR) workflows, from primer design and sample preparation to absolute and relative quantification methods. In today's data-driven biotech landscape, mastering gene expression is a fundamental prerequisite for advanced research in oncology, neuroscience, and infectious diseases. This course focuses heavily on data optimization, troubleshooting experimental flaws, and interpreting high-throughput amplification curves. By integrating theoretical foundations with practical data analysis, you will gain the statistical confidence required to publish in high-impact journals. Transform your laboratory skill set, eliminate trial-and-error bottlenecks, and accelerate your academic or industrial career trajectory in biotechnology.

What you will learn

The core molecular mechanisms behind real-time polymerase chain reaction (rt-PCR and qPCR).Advanced strategies for RNA extraction, quality assessment, and high-fidelity cDNA synthesis.How to design, evaluate, and optimize highly specific primers using digital bioinformatics tools.The explicit differences and applications of SYBR Green dye versus TaqMan probes.Step-by-step methodologies for calculating relative gene expression using the $2^{-\Delta\Delta C_T}$ method.Professional troubleshooting techniques to handle primer-dimers, contamination, and non-specific amplification.

Skills you will gain

RNA Seq Transcriptomics Galaxy Bioinformatics Sequencing Alignment Mapping Quantification Differential Expression Genomics Visualization Annotation Quality Control
Certification

available

Issued by Dr. Omics

Course curriculum

1 module

  • Day 1: Fundamentals of Gene Expression & Primer ArchitectureIntroduction to transcriptomics, MIQE guidelines, and the physics of fluorescence-based detection.Principles of RNA isolation, spectrophotometric quality control, and reverse transcription dynamics.In-silico primer designing workshop: Specificity, melting temperatures ($T_m$), and secondary structures.Day 2: Assay Optimization & qPCR Run ExecutionMaster mix formulation, multiplexing strategies, and reference gene (housekeeping gene) selection.Setting up thermal cycling profiles: Denaturation, annealing extension, and melt curve analysis.Understanding threshold cycles ($C_t$/$C_q$ values) and baseline setups on real-time PCR instruments.Day 3: Data Analytics, Quantitation & TroubleshootingMathematical modeling for gene expression: Relative quantification vs. standard curve absolute quantification.Statistical validation of qPCR datasets, biological replicates vs. technical replicates.Comprehensive troubleshooting clinic: Resolving atypical amplification curves and low efficiency.

What you need to start

  • Basic understanding of molecular biology and genetics concepts.
  • Familiarity with DNA, RNA, and gene expression processes.
  • No prior programming experience required.
  • Access to a computer with a stable internet connection.
  • Basic knowledge of biological data analysis is helpful but not mandatory.
  • Willingness to learn hands-on RNA-Seq data analysis using the Galaxy platform.
  • A modern web browser (Chrome, Firefox, Edge, or Safari).
  • Interest in genomics, transcriptomics, and bioinformatics research.

Who this course is for

  • Life Science Students: Undergraduate and postgraduate students looking to gain industry-ready lab skills.
  • Ph.D. Candidates & Researchers: Scholars aiming to optimize their experimental designs and validate RNA-seq data.
  • Biotech Professionals: Industry technicians looking to upgrade their molecular diagnostic capabilities.
INR

₹199

Billed in INR for all learners, in India and internationally.

Enroll for International Students

Paying from outside India? Use this link to complete your payment.

This course includes
  • Format Recording Available
  • Level All Levels
  • Language English
  • Modules 1
  • Certificate Yes
  • Provider Dr. Omics
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