Webinar Recording Available All Levels Dr. Omics

Essential Molecular Techniques: RNA and cDNA Preparation for RT-PCR Webinar

Master precision wet-lab workflows for high-quality nucleic acid isolation and cDNA synthesis. Learn how predictive AI and optimization tools maximize quantitative gene expression accuracy.

  • 4.5/5
  • English
  • Updated Aug 2026
Essential Molecular Techniques: RNA and cDNA Preparation for RT-PCR Webinar

About this course

Welcome to an essential training program designed to perfect your core laboratory competencies in molecular biology. "Essential Molecular Techniques: RNA and cDNA Preparation for RT-PCR" is a free online international webinar developed by Dr.Omics Edu, as displayed in 21.png. This intensive technical masterclass bridges traditional lab protocols with modern automated advancements, including the use of AI tools for optimal primer design and target simulation. Throughout this session, participants will dive deep into standard operating procedures for isolating high-yield, pure total RNA from diverse biological samples. We will dissect quantitative workflows, contamination troubleshooting, and the chemical mechanics behind reverse transcription into complementary DNA (cDNA). You will learn how to evaluate RNA integrity computationally before initializing downstream quantitative polymerase chain reaction (RT-qPCR) assays. By eliminating common experimental variables and errors, this training provides the fundamental technical capability required for successful academic research and commercial biotechnology diagnostics.

What you will learn

Core laboratory protocols for high-purity RNA extraction and maximizing sample yield stability.
Step-by-step methodologies of the reverse transcription mechanism to generate robust cDNA templates.
How to implement artificial intelligence tools and computational algorithms for precision primer design.
Advanced techniques to identify, calculate, and troubleshoot genomic DNA (gDNA) contamination in your assays.
Best practices for evaluating downstream quantitative RT-PCR results and verifying gene expression signals.

Skills you will gain

RNA-Isolation cDNA-Synthesis RT-PCR Primer-Design Quality-Control Quantitative-Genomics Assay-Optimization Troubleshooting
Certification

Available

Issued by Dr. Omics

Course curriculum

1 module

  • Fundamental biology of RNA molecules, stability challenges, and clean-room laboratory dynamics.
  • Total RNA isolation mechanisms: Column-based extraction versus phenol-chloroform reagents.
  • Quantifying nucleic acids using spectrophotometry, fluorometry, and digital automated profiling.
  • The reverse transcription protocol: Selecting reverse transcriptases, oligo(dT) primers, and random hexamers.
  • Designing optimal qPCR primers using computational intelligence and modern AI web-servers.
  • Troubleshooting common RT-PCR errors, amplicon design pitfalls, and master mix setup strategies.

What you need to start

  • A basic educational background or foundational knowledge in general cell biology, genetics, or molecular concepts.
  • No advanced bioinformatics, coding, or data science skills are necessary to follow along with this wet-lab optimization masterclass.

Who this course is for

  • Undergraduate & Postgraduate Students: Science scholars in biotechnology, biochemistry, or microbiology looking to build concrete laboratory foundational skills.
  • Wet-Lab Research Assistants: Technical staff seeking to standardize their pipetting, extraction, and synthesis workflows to reduce experimental variance.
  • Clinical Diagnostic Professionals: Lab technicians wanting to understand the computational and molecular precision guidelines behind modern diagnostic RT-PCR testing.
INR

₹199

₹999 80% off
USD

$5

$20 75% off

Indian learners pay in INR; international learners are billed in USD.

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Paying from outside India? Use this link to complete your payment.

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This course includes
  • Format Recording Available
  • Level All Levels
  • Language English
  • Modules 1
  • Access 3 months
  • Certificate Yes
  • Provider Dr. Omics
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