About this course
The Four Month: Exome Sequencing and Variant Calling Skill Course at Dr. Omics Edu is a 4-month live, mentor-led online course in NGS and genomics data analysis. Ten modules go deep on exome sequencing and variant calling. You learn on real biological data and finish with a genomics analysis taken from raw reads to interpreted results that you can show to an employer, a guide or an admissions panel.
In this course you move through exome capture and sequencing concepts, quality control and alignment and variant calling and joint genotyping, then apply them in a hands-on project. The course runs live online across 10 modules, so you can ask your mentor questions as you work. It is an advanced-level course, best suited to learners who already know the fundamentals.
What you will learn
• Exome capture and sequencing concepts
• Quality control and alignment
• Variant calling and joint genotyping
• Variant filtering and annotation
• Clinical and population databases
• Prioritising disease-causing variants
Genomics data analyst, NGS bioinformatician, research associate, and a stronger profile for genomics research or higher studies.
Skills you will gain
Linux
BWA
GATK
VEP
ClinVar
samtools
Certification
Certification: A certificate will be awarded upon course completion.
Issued by Dr. Omics Edu
Course curriculum
10 modules
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M1T1 = Introduction to Bioinformatics
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M1T2 = NCBI Database Overview
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M1T3 = Genbank Database Practical Exercises
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M1T4 = UCSC Genome Browser Overview
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M1T5 = UCSC Genome Browser Hands-on Exercises
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M1T6 = Pubmed Database Introduction
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M1T7 = Clinvar Database Overview
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M1T8 = KEGG Database Overview and Exercises
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M1T9 = Protein Databases (UniProt)
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M1T10 = Protein Databases (PDB)
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M1T11 = Online BLAST Introduction and Exercises
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M1T12 = Standalone BLAST Setup and Exercises
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M1T13 = Standalone BLAST Advanced Exercises
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M1T14 = Multiple Sequence Alignment with ClustalW
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M1T15 = Multiple Sequence Alignment with MEGA
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M2T1 = Overview and Installation of Linux
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M2T2 = Basic Linux Commands
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M2T3 = Advanced Linux Commands
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M2T4 = Package Management using Repository
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M2T5 = Package Management using Source Code
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M3T1 = Introduction to Python
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M3T2= Data Types
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M3T3= String Handling
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M3T4= Data Structure
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M3T5=Control Structure
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M3T6 = Function
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M3T7= File Handling
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M3T8= Data Manipulation
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M3T9= Data Visualization
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M3T10= Biopython
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M4T1 = Introduction and Installation of R
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M4T2= Data Types in R
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M4T3= Data Structure
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M4T4= File Handling
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M4T5=Control Structure
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M4T6 = Function
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M4T7= Package Management
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M4T8= Data Manipulation
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M4T9= Data Visualization
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M4T10= Statistical Analysis
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M6T1= Introduction to NGS and DNAseq
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M6T2= Basic Terminologies in NGS
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M6T3= Understanding of SRA database
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M6T4= Tools installation in Linux for Variation Calling
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M6T5= Quality control
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M6T6= Trimming of Reads
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M6T7= Indexing of Genome and Alignment of Reads
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M6T8= Variation calling using GATK
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M6T9= Variant Effect Prediction(VEP)
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M6T10= Variation Visualization (IGV)
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M7T1=Introduction to RNAseq and it’s basic terminologies
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M7T2=Tools installation in Linux for Gene Expression analysis
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M7T3=Quality control and Trimming of reads
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M7T4=Indexing of Genome and Alignment of Reads
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M7T5=Normalization of Data (Cufflinks)
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M7T6=Merging of Data and Differential expression of genes
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M7T7=Understanding of DEG results
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M7T8=Annotation of DEG
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M7T9=Functional and Pathway Enrichment Analysis
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M7T10=Network Analysis
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M8T1= Tools installation for De-novo RNAseq
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M8T2= Tools installation for De-novo RNAseq
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M8T3= Data downlading and Quality control
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M8T4= Assembly Creation
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M8T5= Abundance count estimation
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M8T6= Generation of count matrix and DEG
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M8T7= BLAST
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M8T8= Understanding the DEG results
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M8T9= Annotation of DEGs
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M8T10= Encrichment Analysis
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M9T1= Introduction to metagenomics
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M9T2= Tools installation for metagenomics
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M9T3= Data Downloading
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M9T4= Quality control & Trimming
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M9T5= Data importing in Qimme2
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M9T6= Data quality check using DADA2
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M9T7= Phylogentic Analysis
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M9T8= Taxonomy Analysis
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M9T9= Krona Plot
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M9T0= Phylogenetic tree construction
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M10T1= Introduction to MIcroarray
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M10T2= Introduction to Microarray
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M10T3= Data Downloading
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M10T4= Microarray Pipeline upto Normalization
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M10T5= Microarray Pipeline till DEG
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M10T6= Annotation of DEG
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M10T7= Encrichment Analysis
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M10T8= Network Analysis
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M10T9= Volcano Plot
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M10T10= Heatmap
What you need to start
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Motivation to Learn: A strong desire to engage with and understand the material.
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Basic Knowledge: Familiarity with biology and molecular biology concepts.
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Interest in Technology: Eagerness to learn about the latest technologies in Next-Generation Sequencing (NGS).
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Software: Free software will be utilized, so no additional software purchases are necessary.
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Hardware: A laptop with a minimum of 4GB RAM and 100GB of hard disk space.
Who this course is for
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B.Sc, M.Sc, B.Tech, M.Tech and PhD students in biotechnology, genetics, microbiology, biochemistry or life sciences, and early-career researchers.
