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Instructor Name

Dr.Omics

Category

Research Oriented Courses

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Course Requirements

  • Motivation to Learn: A strong desire to engage with and understand the material.
  • Basic Knowledge: Familiarity with biology and molecular biology concepts.
  • Interest in Technology: Eagerness to learn about the latest technologies in Next-Generation Sequencing (NGS).
  • Software: Free software will be utilized, so no additional software purchases are necessary.
  • Hardware: A laptop with a minimum of 4GB RAM and 100GB of hard disk space

Course Description

  • Foundations of Bioinformatics: Explore genomic bioinformatics, databases, and tools like GenBank and PubMed, bridging biotechnology with bioinformatics.

    • Next Generation Sequencing (NGS): Understand bioinformatics' role in NGS, covering techniques, applications, and Linux basics, including cloud technology.

      • Python and Biopython: Master Python essentials tailored for bioinformatics, with a focus on data manipulation and Biopython for biological sequence analysis.

        • R Programming and Bioconductor: Learn R programming and Bioconductor for statistical analysis and visualization in bioinformatics, enhancing research proficiency.

          • RNA and DNA Sequencing Analysis: Acquire practical skills in RNA and DNA Seq data analysis, including variant detection, annotation, and pathway analysis.

            • Microarray and Metagenome Analysis: Gain expertise in microarray technology, metagenome analysis, and pathway network analysis using advanced tools.

              • HR Session: Develop soft skills and prepare for career advancement through HR sessions covering resume building, interview skills, and professional networking.
              • Research Project and Paper Publication: Apply acquired skills in a two-month NGS research project, leading to paper publication, and enhancing practical proficiency.

              Course Outcomes

              • Gain a comprehensive understanding of bioinformatics fundamentals and their applications in biotechnology research.
              • Master NGS techniques and platforms, coupled with programming skills in Python and R for efficient data analysis.
              • Acquire expertise in utilizing Bioconductor for sequence analysis and visualization, enhancing research proficiency.
              • Develop practical skills in RNA and DNA Seq data analysis, including variant detection and pathway analysis.
              • Enhance proficiency in microarray and metagenome analysis, leading to advanced research capabilities.
              • Apply acquired skills in a two-month NGS research project, leading to paper publication, and prepare for advanced studies and careers in life sciences.
              • Develop a strong understanding of Bioinformatics & NGS basics, Linux, Cloud Computing, Python, and R for NGS applications.

              Rules & Regulations

              • Attendance and Participation: Maintain a minimum of 75% attendance. Regular assessments and attendance contribute to performance evaluation.
              • Discipline: Maintain punctuality and respect in live classes. Engage actively and interact respectfully with instructors and peers.
              • Course Fee Payment: Pay course fees on time to avoid suspension or cancellation of access.
              • Assignments and Project: Complete assignments and projects sincerely and submit them on time.
              • Feedback and Communication: Maintain open communication with instructors and provide constructive feedback.
              • Certification: A certificate will be awarded upon course completion.
              • Paper Publication: eligibility requires a minimum 75% combined attendance and performance score.


              Course Curriculum DOWNLOAD BROCHURE

              1 Introduction to Bioinformatics
              1 Hour


              2 NCBI Database Overview
              1 Hour


              3 Genbank Database Practical Exercises
              1 Hour


              4 UCSC Genome Browser Overview
              1 Hour


              5 UCSC Genome Browser Hands-on Exercises
              1 Hour


              6 Pubmed Database Introduction
              1 Hour


              7 Clinvar Database Overview
              1 Hour


              8 KEGG Database Overview and Exercises
              1 Hour


              9 Protein Databases (UniProt)
              1 Hour


              10 Protein Databases (PDB)
              1 Hour


              11 Online BLAST Introduction and Exercises
              1 Hour


              12 Standalone BLAST Setup and Exercises
              1 Hour


              13 Standalone BLAST Advanced Exercises
              1 Hour


              14 Multiple Sequence Alignment with ClustalW
              1 Hour


              15 Multiple Sequence Alignment with MEGA
              1 Hour


              1 Overview and Installation of Linux
              1 Hour


              2 Basic Linux Commands
              1 Hour


              3 Advanced Linux Commands
              1 Hour


              4 Package Management using Repository
              1 Hour


              5 Package Management using Source Code
              1 Hour


              1 Introduction to Python
              1 Hour


              2 Data Types
              1 Hour


              3 String Handling
              1 Hour


              4 Data Structure
              1 Hour


              5 Control Structure
              1 Hour


              6 Function
              1 Hour


              7 File Handling
              1 Hour


              8 Data Manipulation
              1 Hour


              9 Data Visualization
              1 Hour


              10 Biopython
              1 Hour


              1 Introduction and Installation of R
              1 Hour


              2 Data Types in R
              1 Hour


              3 Data Structure
              1 Hour


              4 File Handling
              1 Hour


              5 Control Structure
              1 Hour


              6 Function
              1 Hour


              7 Package Management
              1 Hour


              8 Data Manipulation
              1 Hour


              9 Data Visualization
              1 Hour


              10 Statistical Analysis
              1 Hour


              1 Introduction to NGS and DNAseq
              1 Hour


              2 Basic Terminologies in NGS
              1 Hour


              3 Understanding of SRA database
              1 Hour


              4 Tools installation in Linux for Variation Calling
              1 Hour


              5 Quality control
              1 Hour


              6 Trimming of Reads
              1 Hour


              7 Indexing of Genome and Alignment of Reads
              1 Hour


              8 Variation calling using GATK
              1 Hour


              9 Variant Effect Prediction(VEP)
              1 Hour


              10 Variation Visualization (IGV)
              1 Hour


              1 Introduction to RNAseq and it’s basic terminologies
              1 Hour


              2 Tools installation in Linux for Gene Expression analysis
              1 Hour


              3 Quality control and Trimming of reads
              1 Hour


              4 Indexing of Genome and Alignment of Reads
              1 Hour


              5 Normalization of Data (Cufflinks)
              1 Hour


              6 Merging of Data and Differential expression of genes
              1 Hour


              7 Understanding of DEG results
              1 Hour


              8 Annotation of DEG
              1 Hour


              9 Functional and Pathway Enrichment Analysis
              1 Hour


              10 Network Analysis
              1 Hour


              1 Tools installation for De-novo RNAseq
              1 Hour


              2 Tools installation for De-novo RNAs eq
              1 Hour


              3 Data downlading and Quality control
              1 Hour


              4 Assembly Creation
              1 Hour


              5 Abundance count estimation
              1 Hour


              6 Generation of count matrix and DEG
              1 Hour


              7 BLAST
              1 Hour


              8 Understanding the DEG results
              1 Hour


              9 Annotation of DEGs
              1 Hour


              10 Encrichment Analysis
              1 Hour


              1 Introduction to metagenomics
              1 Hour


              2 Tools installation for metagenomics
              1 Hour


              3 Data Downloading
              1 Hour


              4 Quality control & Trimming
              1 Hour


              5 Data importing in Qimme2
              1 Hour


              6 Data quality check using DADA2
              1 Hour


              7 Phylogentic Analysis
              1 Hour


              8 Taxonomy Analysis
              1 Hour


              9 Krona Plot
              1 Hour


              10 Phylogenetic tree construction
              1 Hour


              1 Introduction to MIcroarray
              1 Hour


              2 Introduction to MIcroarray
              1 Hour


              3 Data Downloding
              1 Hour


              4 Microarray Pipeline upto Normalization
              1 Hour


              5 Microarray Pipeline till DEG
              1 Hour


              6 Annotation of DEG
              1 Hour


              7 Encrichment Analysis
              1 Hour


              8 Network Analysis
              1 Hour


              9 Volcano Plot
              1 Hour


              10 Heatmap
              1 Hour


              Student Feedback

              NGS RESEARCH ORIENTED COURSE ( 6 MONTH )

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