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Next Generation Sequencing : Variant Calling Analysis

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Course Requirements

  • Motivation to Learn: A strong desire to engage with and understand the material.
  • Basic Knowledge: Familiarity with biology and molecular biology concepts.
  • Interest in Technology: Eagerness to learn about the latest technologies in Next-Generation Sequencing (NGS).
  • Software: Free software will be utilized, so no additional software purchases are necessary.
  • Hardware: A laptop with a minimum of 4GB RAM and 100GB of hard disk space.

Course Description

  • Introduction to the fundamentals of Next-Generation Sequencing (NGS) and its role in genetic research.
  • Explore the principles and workflows of variant calling, including SNPs and indels.
  • Hands-on training in processing raw sequencing data using tools like FASTQC and Trimmomatic.
  • Learn to align sequencing data with reference genomes using tools such as BWA and Bowtie2.
  • Perform variant calling and annotation with industry-standard software like GATK and ANNOVAR.
  • Interpret and validate identified variants to derive meaningful biological insights.


Course Outcomes

  • Understand the basics of NGS and its applications in genomics and personalized medicine.
  • Gain proficiency in quality control and pre-processing of NGS data.
  • Perform sequence alignment and evaluate the accuracy of mapping results.
  • Execute variant calling workflows to identify SNPs and indels in sequencing data.
  • Annotate and interpret variants using bioinformatics tools and databases.
  • Develop skills to apply variant analysis in research, diagnostics, or personalized genomics projects.

Rules & Regulations

  • Attendance: Students must watch at least 75% of the recorded sessions to meet the minimum participation criteria.
  • Feedback and Communication: Maintain open communication with instructors for clarifications and actively provide constructive feedback to enhance the course experience.
  • Certification: A certificate of completion will be awarded to students who fulfill the attendance requirement and complete all necessary course components.

Course Curriculum

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Super admin
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