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Super admin

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Course Requirements

  • Good Internet connection

Course Description

This webinar begins with an Introduction to Essential Molecular Techniques, where participants will learn the fundamental concepts and methodologies involved in RNA and cDNA preparation for RT-PCR. Following this, the RNA Extraction and Purification session will cover key laboratory techniques for isolating high-quality RNA, ensuring accuracy in downstream applications. The cDNA Synthesis and Quality Assessment module will provide hands-on insights into reverse transcription methods and strategies to assess cDNA integrity. Next, the RT-PCR Optimization and Troubleshooting section will focus on optimizing reaction conditions, addressing common challenges, and ensuring reliable results. Finally, the webinar concludes with a discussion on Advanced Applications and Future Trends, exploring cutting-edge developments in molecular techniques and their impact on research and clinical diagnostics

Course Outcomes

  • Introduction – Overview of RT-PCR and key topics.
  • Fundamentals of RNA Preparation – Importance of RNA quality and integrity.
  •  cDNA Synthesis – Key steps in reverse transcription.
  •  RT-PCR Master Mix Preparation – Essential components and contamination prevention.
  •  RT-PCR Amplification & Data Analysis – Understanding Ct values and troubleshooting.
  •  Your RT-PCR Toolkit – Must-have reagents, enzymes, and analysis tools.
  •  RT-PCR Applications & Advances – Use in research, diagnostics, and emerging techniques.
  •  Key Takeaways – Summary of best practices and optimization tips.
  • Open Q&A Session – Addressing participant questions and challenges.


Rules & Regulations

  •  Attendance and Participation: Attendance is mandatory to ensure a comprehensive learning experience.
  •  Discipline: Maintain punctuality and respect in the sessions. Engage actively and interact respectfully with instructors and peers.
  • Certification: A certificate will be awarded upon webinar completion.

Course Curriculum

Instructor

Administrator

Super admin

Administrator

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book.

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Essential Molecular Techniques: RNA and cDNA Preparation for RT-PCR

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