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DNA Seq Data Analysis Recorded Course

Instructor Name

Super admin
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Core Courses

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Course Requirements

  • Motivation to Learn: A strong desire to engage with and understand the material.
  • Basic Knowledge: Familiarity with biology and molecular biology concepts.
  • Interest in Technology: Eagerness to learn about the latest technologies in Next-Generation Sequencing (NGS).
  • Software: Free software will be utilized, so no additional software purchases are necessary.
  • Hardware: A laptop with a minimum of 4GB RAM and 100GB of hard disk space.

Course Description

  • Introduction to variant calling tools and methods, focusing on installation and usage.
  • Comprehensive coverage of DNA sequencing data handling, including retrieval, quality assessment, and mapping to a reference genome.
  • Exploration of genetic variation detection and visualization techniques for comprehensive analysis.
  • Annotation and prediction of variant effects, with a specific focus on non-synonymous mutations and their impact on protein function.
  • Overview of RNA sequencing, including tool installation, data preprocessing, and mapping reads to a reference genome.
  • Hands-on experience with visualization techniques for gene expression analysis and pathway network analysis using KEGG database.

Course Outcomes

  • Gain proficiency in variant calling and annotation techniques for DNA sequencing data.
  • Master the steps involved in RNA sequencing data analysis, including quality assessment and preprocessing.
  • Acquire practical skills in mapping reads to a reference genome and interpreting mapping output.
  • Understand the principles of gene expression quantification and pathway analysis using RNA sequencing data.
  • Develop the ability to perform pathway network analysis and interpret biological pathways involved in gene expression regulation.

Rules & Regulations

  •  Attendance and Participation: Maintain a minimum of 75% attendance. Regular assessments and attendance contribute to performance evaluation.
  •  Discipline: Maintain punctuality and respect in live classes. Engage actively and interact respectfully with instructors and peers.
  • Course Fee Payment: Pay course fees on time to avoid suspension or cancellation of access.
  • Assignments and Project: Complete assignments and projects sincerely and submit them on time.
  • Feedback and Communication: Maintain open communication with instructors and provide constructive feedback.
  • Certification: A certificate will be awarded upon course completion.

Course Curriculum

1 1) Introduction to DNAseq and FASTQ format
50 Min

2 2)Installation of tools
49 Min

3 3) Quality control of samples(FastQC)
49 Min

4 4)Understanding of Important terminologies, Data searching and Data Downloading from SRA database
50 Min

5 5)Variation Visualization
50 Min

6 6)Variation Annotation
50 Min

7 7)Understanding of VCF file format and Variation Filtration
50 Min

8 8)Variation Calling
49 Min

9 9)Indexing of Genome & Mapping of reads to reference genomeBWABowtie
50 Min

10 Trimming of dataTrimomaticcutadaptfastp
50 Min

Instructor

Administrator

Super admin
Administrator

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book.

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30 Students
27 Courses

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.

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This course includes:

  • Duration

  • Skill Level Beginners

  • Lectures 10 lessons

  • Full lifetime access

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