Dr. Omics Education;

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Course Requirements

Motivation to Learn: A strong desire to engage with and understand the material.
Basic Knowledge: Familiarity with biology and molecular biology concepts.
Interest in Technology: Eagerness to learn about the latest technologies in Next-Generation Sequencing (NGS).
Software: Free software will be utilized, so no additional software purchases are necessary.
Hardware: A laptop with a minimum of 4GB RAM and 100GB of hard disk space.

Course Description

Introduction to CRISPR technology, including its principles and applications in gene editing.
Overview of CRISPR experimental design, including guide RNA design, delivery, and validation strategies.
Learn the methodologies for analyzing CRISPR-generated mutations such as insertions, deletions, and point mutations.
Gain hands-on experience with CRISPR data analysis tools, including CRISPR, Cas-Analyzer, and other bioinformatics software.
Explore techniques for evaluating editing efficiency, specificity, and potential off-target effects.
Develop skills to interpret CRISPR experimental outcomes and assess their biological relevance.

Course Outcomes

Understand the fundamental principles and applications of CRISPR gene-editing technology.
Design CRISPR experiments, including guide RNA design, delivery methods, and validation techniques.
Analyze CRISPR-induced mutations and interpret sequencing data for accurate mutation detection.
Use bioinformatics tools to evaluate editing efficiency and identify off-target effects.
Assess the quality and specificity of CRISPR edits using various data analysis methods.
Interpret CRISPR data for applications in functional genomics, gene therapy, and biomedical research.

Rules & Regulations

Attendance: Students must watch at least 75% of the recorded sessions to meet the minimum participation criteria.
Feedback and Communication: Maintain open communication with instructors for clarifications and actively provide constructive feedback to enhance the course experience.
Certification: A certificate of completion will be awarded to students who fulfill the attendance requirement and complete all necessary course components.

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